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. 2017 Jun 21;7:3978. doi: 10.1038/s41598-017-04432-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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U94A expression inhibits hOPC migration in vivo but does not prevent oligodendrocyte differentiation. (a) hOPCs expressing U94A (RFP) or controls (GFP) were injected bilaterally into the corpus callosum and hippocampus of cuprizone-treated NSG mice. Brains were analyzed 21 days after transplantation. Representative confocal images from (b) corpus callosum white matter and (c) hippocampus. (d) U94A RFP+ hOPCs migrated significantly shorter distances than GFP+ controls. Cell dispersion was measured using stereological measurements of the distance of each cell to the center point of the transplanted cell population. Measurements averaged from at least three different sections from each of four different animals. (e) Analysis of NSG brains at 21 days post- transplant show that a majority of GFP+ and U94A RFP+ hOPCs differentiated into GSTπ positive oligodendrocytes (arrowheads). (f) No GFP+ or U94A RFP+ hOPCs were observed to differentiate into GFAP+ astrocytes (asterisks). Cell dispersion data displayed as mean ± SEM; *p < 0.05 versus GFP+ control. Effect of U94A expression on hOPC cell dispersion was analyzed by unpaired student’s t-test.