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. 2017 Jun 21;7:4005. doi: 10.1038/s41598-017-04198-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Intrathecal injection of CXCR4 siRNA suppresses TCI-induced up-regulation of NMDAR1 expression in spinal neurons. (A) Immunofluorescence staining showing that NMDAR1 (green) co-localized with neurons (NeuN, red), but not astrocytes (GFAP, red) or microglial cells (IBA1, red). (B) CXCR4 (red) co-expressed with NMDAR1 (green) in the spinal cord. Tissues were taken on day 14 after TCI. Original magnification: 400× (A and B), and 1600 × (inserts in A and B); scale bar: 50 μm (A and B), and 20 μm (inserts in A and B). (C and D) Western blot and RT-qPCR analyses showing the inhibitory effect of CXCR4 siRNA on TCI-induced over-expression of NMDAR1 protein (C) and mRNA (D) on day 14 after TCI. CXCR4 siRNA (siRNA, 5 μg/10 μl) or scrambled siRNA (Scr, 5 μg/10 μl) were administered once daily from days 12 to 14. L4-L6 spinal tissues were collected 12 hours after the last injection. **P < 0.01 vs sham group; ^# P < 0.05, ^## P < 0.01 vs TCI group; n = 4 for each group.