Figure 5.

GSH-QDs applied in a step-wise manner, before or after DNFB application. (a) We applied the GSH-QDs to the mouse ear for 1 hour, then wiped the majority of the GSH-QDs off with a PBS soaked cotton swab before the DNFB challenge dose was applied. DNFB application to the wiped ear has a reduced swelling response (1 Hour NP + 0.2% DNFB). (b) DNFB was applied to the ear first for 1 hour followed by GSH-QD application. The ear swelling response was reduced. Mean ± SEM, *p < 0.05, Student’s 2-tailed t-Test, paired with unequal variances, N = 3–4.