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. 2017 Jun 21;7:3979. doi: 10.1038/s41598-017-03729-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mast cells quantified in the ear tissue by IHC using the Geimsa stain. The number of degranulated MCs was significantly lower in the silica NP (20 nm) treatment group compared to PEI-QD and CNT treated ears co-challenged ear. MCs were counted in 10 fields of view at 40X for 3 independent mouse skin samples. Mean ± SEM, *p < 0.05, 2-tailed Student’s t-Test, paired, with respect to intact mast cells in each group. Error Bars: Standard Dev.