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. 2017 Jun 21;7:3974. doi: 10.1038/s41598-017-04322-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PSTreg express high amounts of IL-10 and TGF-β1. Supernatants from Treg and Teff cocultures were harvested after 7 h and 96 h. IL-10 (a), TGF-β1 (b) and IFN-γ (c) were quantified with BD™ CBA Flex Set analysis and ELISA. Data represents 4-14 independent experiments from 4–8 donors. ***p < 0.001, **p < 0.01, *p < 0.05, NS, non-significant, p > 0.05. ANOVA with Bonferroni’s Multiple Comparison Test was used to determine the statistical significance. The cells of the functional assay were also harvested after coculture for 96 h, and RNA was isolated and the indicated mRNA was quantified by RT-PCR. Relative expression of IL-10, TGFB1 (d) and IL-35 related mRNA (e) in PP to other groups was shown, PN, NP, NN as control. Data represents 7 independent experiments from 6 donors. ACTB, G6PDH or CYPB mRNA were determined from the same sample and used as endogenous reference genes. Wilcoxon signed-rank test was used to determine the p values.