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Maturation cocktail for generation of tolDC and C5-DC.
| TolDC | C5-DC | |
|---|---|---|
| GM-CSF (Leukine sargramostim, USA) | 100 ng/ml | 100 ng/ml |
| IL-4 (R&D, USA) | 20 ng/ml | 20 ng/ml |
| IL-1β (R&D, USA) | 10 ng/ml | 10 ng/ml |
| TNF-α (PEPRO-TECH, USA) | 20 ng/ml | 20 ng/ml |
| PGE2 (Sigma, USA) | 250 ng/ml | 1 µg/ml |
| R848 (In vivo Gen) | — | 1 µg/ml |
| IFN-γ (Boehringer Ingelheim, Germany) | — | 5000 U/ml |
| IL-6 (R&D, USA) | 15 ng/ml | — |
| TGF-β1 (PEPRO-TECH, USA) | 1 ng/ml | — |
| IL-10 (PEPRO-TECH, USA) | 20 ng/ml | — |
