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. 2017 Jun 21;7:3981. doi: 10.1038/s41598-017-04268-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Nanoallergens reveal crucial epitopes in a clinical population. Four patient sera were tested for degranulation response with nanoallergens presenting Ara h2 epitopes. (A) EC₅₀ values of the degranulation assays are shown. Note that epitope 4 and 8 did not demonstrate any degranulation in any of the patient sera. A’s indicate a EC₅₀ values between 5000–2500 pM, B’s indicate a EC₅₀ values > 5000pM. (B) D_max values for degranulation assays.