Table 3.

Kinetic parameters of the Tm1743 double mutants.

Enzymes	K m (mM)	k cat (s−1)	k cat/K m (s −1mM−1)
Wild-type	1.10 ± 0.04	1.96 ± 0.02	1.78 ± 0.03
W21L/W118E	10.53 ± 0.10	1.98 ± 0.02	0.19 ± 0.01
W21L/W118H	15.98 ± 0.20	1.98 ± 0.01	0.12 ± 0.01
W21S/W86E	1.25 ± 0.02	1.01 ± 0.01	0.81 ± 0.02
W21S/W86N	1.62 ± 0.02	0.84 ± 0.01	0.51 ± 0.02