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. 2017 Jun 21;7:3950. doi: 10.1038/s41598-017-04158-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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hiPSC aggregates cultured in suspension with SC and PC commercial media. Light microscopy images of (a) SC TeSR-E8, (b) SC mTeSR1, and (c) SC StemMACS iPS-Brew XF showed transparent media, while (d) PC TeSR-E8, (e) PC mTeSR1, and (f) PC StemMACS iPS-Brew XF were all characterized by particle precipitation. Concerning the hiPSC aggregate culture, (g) low protein TeSR-E8 supported the culture when static conditioned, but (j) it caused hiPSC disruption when peristaltic pump conditioned. Contrarily, static and peristaltic pump conditioning of high protein media supported hiPSC aggregate culture, even if with (h,k) hiPSC aggregates more heterogeneous in size in case of mTeSR1, and (i,l) elevated amount of particles in case of StemMACS iPS-Brew XF. Scale bar = 100 µm.