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. 2017 Jun 21;7:3976. doi: 10.1038/s41598-017-04274-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Identification of HCV E1E2-derived peptides with antiviral activity against HCVcc infection. (A) Effects of E1E2-derived peptides on HCV E1E2-mediated infection. HCVcc Jc1P7NS2Gluc2a was used to screen for antiviral activity. HCVcc was incubated with 10 nM peptides for 1 h prior to addition to Huh7.5/CD81 cells, and Gaussia luciferase activity was assayed 72 h later. The results were normalized to DMSO-treated cells. (B) Characterization of the specificity of inhibitory peptides against HCVcc infection. HCVcc and VSV-Gpp (VSV-G-pseudotyped lentivirus) were incubated with 10 nM peptides for 1 h. Then, Huh7.5/CD81 cells were inoculated for 4 h with virus/peptide mixtures, and Gaussia luciferase activity was assayed 72 h later. IFN-α and anti-CD81 antibodies were used as positive controls, and DMSO was used as a negative control. (C) The ED50 values of peptides were calculated based on their inhibition of HCVcc activity at different peptide concentrations. (D) Cytotoxicity of peptides against Huh7.5/CD81 cells by MTT assay. Each bar represents the average of triplicate data points, with the standard deviation indicated by the error bars.