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. 2017 Jun 21;7:3976. doi: 10.1038/s41598-017-04274-8

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of the combination effects of the peptides combined with IFN-α2b and anti-CD81 antibodies. (A) Determination of the EC50 of IFN-α2b and anti-CD81 antibodies for HCVcc. Huh7.5/CD81 cells were seeded at 1 × 10⁴/well in 96-well plates the day before infection. The cells were infected with 0.05 FFU/cell mixed with serial dilutions of IFN-α2b and anti-CD81 antibodies. Gaussia luciferase activity was measured 72 h later. DMSO treatment served as a negative control. (B,C,D) The inhibitory effects of the peptides in combination with IFN-α2b or anti-CD81 antibody against HCV infection was assayed with chimeric HCVcc. Huh7.5/CD81 cells were treated with peptides, IFN-α2b or anti-CD81 antibodies and infected with different genotypes of chimeric HCVcc. The Gaussia luciferase activities of Jc1P7NS2Gluc2a (B), H77/JFHGluc2a (C), HB/JFHGluc2a (D) were assayed 72 h later. HCV infectivity was expressed as the percentage of the DMSO control. Each bar represents the average of triplicate data points, with the standard deviation represented by the error bar.