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. 2017 Jun 21;7:3945. doi: 10.1038/s41598-017-04233-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Nrf2 protein expression in HepG2 cells is enhanced by chlorophyllin sodium copper salt and bonaphton. HepG2 cells were treated with the two compounds for 5 h. Sulforaphane, a known Nrf2 activator, was used as a positive control (5 μM; PC). Total cell lysates were immunoblotted using the Nrf2 antibody. (A) Chlorophyllin sodium copper salt (100 μM) increased Nrf2 protein expression. (B) Bonaphton (100 μM) increased Nrf2 protein expression. Values are presented as the mean ± S.E.M. *p < 0.05 vs. vehicle-treated cells, t-test (n = 4 per experimental group). Experiments were repeated four times with consistent results, and a representative blot is shown. Full-length blots are presented in Supplementary Fig. S4.