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. 2017 Jun 21;7:3984. doi: 10.1038/s41598-017-04409-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Aggregation pathway for BSA aggregation at 70 °C. M_N and M_U are the native and unfolded forms of monomeric BSA, respectively. D is a dimer, which is a product of slow dimerization of M_U. D₂, D_i, D_i+1, D_j and D_i+j are aggregates formed by dimers D (subscript is a number of dimers).