Figure 3.

Differentially expressed Ft proteins are immunogenic in multiple models of infection. Whole cells (WC) lysates of Ft LVS grown in MHB (M), BHI (B), or BCA (BC) were partitioned into soluble and membrane fractions prior to SDS-PAGE (A) and western blot analysis (B–D). Each lane was loaded with material derived from 1^*10e8 Ft. Asterisks in (A) indicate 2 examples of coomassie-visable, differentially-abundant proteins, one soluble ~23 kDa and one ~120 kDa membrane. Replicate blots were independently probed with sera from S4-convalescent, inbred (C57/BL/6) mice; a S4-convalescent, outbred rabbit; and an LVS-vaccinated human. Asterisks in (B–D) indicate two examples of differentially-detected moieties, one soluble ~23 kDa and one ~120 kDa membrane.