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. 2017 Jun 22;8:1107. doi: 10.3389/fpls.2017.01107

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Copyright © 2017 Feng, Yin and Fei.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of BdVRN genes in meristems of the non-vernalization-requiring Bd 21 and vernalization-requiring Bd29-1 accessions. (A) BdVRN gene expression in meristems of 4-week-old seedlings of Bd29-1 that were vernalized for 2, 4, 6, 8, 10, or 12 weeks. BdGAPDH was used as loading control. (B–D) Relative expression levels of BdVRN1, BdVRN2, and BdVRN3 genes in meristems of Bd21 and Bd29-1. Prior to vernalization, plants were grown at a greenhouse (25°C, 16/8 h day/night). Four-week-old seedlings were transferred to a growth chamber at 4°C and 8/16 h (day/night) for vernalization treatment. Meristems were harvested after 2, 4, 6, 8, 10, or 12 weeks of vernalization treatment. Error bars represent the standard deviation from independent plants.