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. 2017 Jun 22;8:1107. doi: 10.3389/fpls.2017.01107

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Copyright © 2017 Feng, Yin and Fei.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Wild type (WT) (left) and BdVRN1 RNAi mutant plant (right) 7 weeks after seed germination. (B) Real-Time RT-PCR analysis of the BdVRN1 expression level in the WT, knockdown mutants of BdVRN1 RNAi-4 and RNAi-12. (C) Average number of days required for flowering for the WT Bd21 and RNAi mutants with or without a 6-week vernalization treatment. Four-week-old seedlings (30 plants for each accession) were either not vernalized or vernalized for 6 weeks at 4°C and 8/16 h (day/night) in a growth chamber. After the vernalization treatment, plants were transferred to a greenhouse (25°C, 16/8 h day/night). NF denotes non-flowering phenotype. The average number of days to flowering indicates the number of days passed before flowering occurs following a vernalization treatment. Error bars represent the standard deviation from independent plants.