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. 2017 Jun 22;8:1107. doi: 10.3389/fpls.2017.01107

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Copyright © 2017 Feng, Yin and Fei.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression analysis of BdVRN1 (A), BdVRN2 (B), and BdVRN3 (C) genes in the WT (Bd21) and BdVRN1 RNAi mutant. The relative expression levels of each gene were determined by Real-Time RT-PCR in leaf tissues. Prior to vernalization, plants were grown at a greenhouse (25°C, 16/8 h day/night). Four-week-old seedlings were then transferred to a growth chamber at 4°C (8/16 h day/night) for 0 or 6 weeks. BdGAPDH is a loading control. Error bars represent the standard deviation from independent plants.