TABLE 2 .
Heparin-binding proteins identified by mass spectrometry
| Fraction and ORF | Proteina | Peptide countb | Spectrum countc | % coveraged | Heparin-binding motife |
|---|---|---|---|---|---|
| Secreted fraction | |||||
| SAUSA300_0955 | Autolysin | 34 | 137 | 40 | None |
| SAUSA300_0113 | Immunoglobulin G-binding protein A | 22 | 31 | 45 | None |
| SAUSA300_0032 | Penicillin-binding protein 2′ | 17 | 53 | 34 | MKKIKIAKKFHL |
| SAUSA300_1058 | Alpha-hemolysin | 18 | 39 | 55 | NHNKKL |
| SAUSA300_0760 | Enolase | 6 | 17 | 27 | None |
| SAUSA300_1150 | Elongation factor Ts | 12 | 5 | 16 | GRLRKY |
| SAUSA300_0756 | Glyceraldehyde-3-phosphate dehydrogenase | 2 | 12 | 25 | None |
| SAUSA300_0533 | Elongation factor Tu | 6 | 22 | 51 | None |
| SAUSA300_2436 | Surface protein G (putative cell wall surface anchor family protein) | 17 | 32 | 15 | LKRFHSRKN |
| SAUSA300_0055 | Zinc-dependent alcohol dehydrogenase | 2 | 23 | 51 | None |
| SAUSA300_2579 | N-acetyl-muramoyl-l-alanine amidase | 8 | 17 | 17 | None |
| SAUSA300_1790 | Foldase protein PrsA precursor | 8 | 14 | 23 | VKSKKS |
| SAUSA300_0602 | Uncharacterized protein | 12 | 22 | 60 | None |
| SAUSA300_0307 | Lipoprotein family 5′-nucleotidase | 9 | 51 | 35 | IKKNKGAKKSHI |
| SAUSA300_0993 | Pyruvate dehydrogenase E1 component alpha subunit | 3 | 11 | 29 | VRFRKF |
| SAUSA300_1975 | Aerolysin/leukocidin family protein | 12 | 30 | 41 | None |
| SAUSA300_1974 | Leukocidin/hemolysin | 7 | 15 | 28 | VHYKRS |
| SAUSA300_0536 | Molecular chaperone Hsp31 and glyoxalase 3 | 2 | 13 | 31 | None |
| SAUSA300_2079 | Fructose bisphosphate aldolase | 3 | 4 | 16 | None |
| SAUSA300_1362 | DNA-binding protein HU | 3 | 12 | 56 | None |
| SAUSA300_0099 | 1-Phosphatidylinositol phosphodiesterase | 7 | 7 | 21 | None |
| SAUSA300_0994 | Pyruvate dehydrogenase E1 component beta subunit | 4 | 11 | 43 | None |
| SAUSA300_1382 | LukS | 4 | 7 | 17 | None |
| SAUSA300_1381 | LukF | 7 | 9 | 30 | AKKSKI |
| SAUSA300_0618 | ABC transporter substrate-binding protein | 10 | 5 | 16 | None |
| SAUSA300_2573 | IsaB | 6 | 14 | 30 | None |
| SAUSA300_2199 | 50S ribosomal protein L22 | 2 | 8 | 57 | LKRFRPPRKVRL |
| SAUSA300_2189 | 50S ribosomal protein L6 | 2 | 7 | 29 | None |
| SAUSA300_2506 | Probable transglycosylase IsaA | 5 | 12 | 35 | None |
| SAUSA300_1603 | 50S ribosomal protein L21 | 4 | 3 | 39 | None |
| SAUSA300_1920 | Chemotaxis inhibitory protein | 4 | 7 | 27 | THHHSAKA |
| SAUSA300_2177 | 50S ribosomal protein L17 | 2 | 5 | 30 | None |
| SAUSA300_1052 | Fibrinogen-binding protein | 3 | 3 | 21 | FKRTRTTHRKAQRA |
| SAUSA300_0964 | Chitinase | 2 | 2 | 18 | None |
| SAUSA300_2196 | 50S ribosomal protein L29 | 3 | 2 | 32 | None |
| SAUSA300_0801 | Enterotoxin, Seq | 3 | 3 | 18 | NKTKKGIKLRKY |
| SAUSA300_0883 | Putative surface protein | 4 | 5 | 42 | None |
| SAUSA300_2195 | 30S ribosomal protein S17 | 2 | 2 | 24 | YKTHKLGKRVKYSKKYKT |
| SAUSA300_0994 | Putative pyruvate dehydrogenase E1 beta subunit | 3 | 7 | 65 | None |
| SAUSA300_2164 | Surface protein | 2 | 2 | 24 | None |
| Cell wall fraction | |||||
| SAUSA300_0113 | Immunoglobulin G-binding protein A | 21 | 31 | 45 | None |
| SAUSA300_2192 | 50S ribosomal protein L5 | 5 | 5 | 39 | None |
| SAUSA300_0760 | Enolase | 16 | 17 | 27 | None |
| SAUSA300_1150 | Elongation factor Ts | 13 | 5 | 16 | GRLRKY |
| SAUSA300_0756 | Glyceraldehyde-3-phosphate dehydrogenase | 15 | 12 | 25 | None |
| SAUSA300_0533 | Elongation factor Tu | 13 | 22 | 51 | None |
| SAUSA300_0055 | Zinc-dependent alcohol dehydrogenase | 15 | 23 | 51 | None |
| SAUSA300_0220 | Formate acetyltransferase | 13 | 16 | 21 | LRSHKTYRKTHN |
| SAUSA300_2540 | Fructose-bisphosphate aldolase class I | 15 | 17 | 43 | None |
| SAUSA300_1644 | Pyruvate kinase | 12 | 16 | 28 | LKNKKGMRKTKI |
| SAUSA300_0757 | Phosphoglycerate kinase | 11 | 6 | 19 | None |
| SAUSA300_1540 | Chaperone protein DnaK | 13 | 9 | 15 | None |
| SAUSA300_0235 | l-Lactate dehydrogenase | 20 | 7 | 36 | None |
| SAUSA300_2198 | 30S ribosomal protein S3 | 6 | 8 | 36 | LKIRKF |
| SAUSA300_0532 | Elongation factor G | 15 | 16 | 32 | IKKNKG|SRRGRVGRIHKI |
| SAUSA300_1201 | Glutamine synthetase | 10 | 9 | 16 | ARKHNLHA |
| SAUSA300_1640 | Isocitrate dehydrogenase | 5 | 7 | 18 | None |
| SAUSA300_2362 | 2,3-Bisphosphoglycerate-dependent phosphoglycerate mutase | 8 | 20 | 52 | None |
| SAUSA300_0536 | Molecular chaperone Hsp31 | 10 | 13 | 31 | None |
| SAUSA300_1138 | Succinyl-CoA synthetase, beta subunit | 11 | 7 | 18 | None |
| SAUSA300_1149 | 30S ribosomal protein S2 | 5 | 10 | 18 | PKMKKY |
| SAUSA300_0186 | Phosphate acetyltransferase | 10 | 11 | 41 | None |
| SAUSA300_1365 | 30S ribosomal protein S1 | 13 | 7 | 15 | None |
| SAUSA300_1641 | Citrate synthase II, GltA | 10 | 7 | 15 | None |
| SAUSA300_0523 | 50S ribosomal protein L1 | 4 | 13 | 28 | None |
| SAUSA300_2078 | UDP-N-acetylglucosamine 1-carboxyvinyltransferase | 5 | 5 | 17 | GRFKKC |
| SAUSA300_1331 | Alanine dehydrogenase | 5 | 5 | 15 | None |
| SAUSA300_0973 | Phosphoribosylformylglycinamide cycloligase | 6 | 7 | 22 | None |
| SAUSA300_2362 | 2,3-Bisphosphoglycerate-independent phosphoglycerate mutase | 10 | 13 | 24 | None |
| SAUSA300_0389 | GMP synthase | 7 | 7 | 20 | IKSHHN |
| SAUSA300_2178 | DNA-directed RNA polymerase | 9 | 11 | 36 | None |
| SAUSA300_0129 | 2-Butanediol dehydrogenase | 8 | 19 | 50 | None |
| SAUSA300_1239 | Transketolase | 9 | 9 | 21 | None |
| SAUSA300_0539 | Branched-chain amino acid transferase | 8 | 11 | 23 | None |
| SAUSA300_0758 | Triose phosphate isomerase | 11 | 20 | 56 | None |
| SAUSA300_1666 | 30S ribosomal protein S4 | 4 | 6 | 25 | ARTRRQ |
| SAUSA300_0871 | Fumarylacetoacetate hydrolase | 7 | 7 | 35 | None |
| SAUSA300_0886 | 3-Oxoacyl-synthase 2 | 8 | 10 | 33 | None |
| SAUSA300_0067 | Universal stress protein | 4 | 10 | 34 | None |
| SAUSA300_1491 | Xaa-Pro dipeptidase | 5 | 7 | 16 | None |
| SAUSA300_1804 | Glucosamine-6-phosphate isomerase | 7 | 8 | 16 | None |
| SAUSA300_1622 | Trigger factor | 5 | 6 | 15 | None |
| SAUSA300_0966 | N5-carboxyaminoimidazole ribonucleotide | 6 | 5 | 39 | None |
| SAUSA300_2092 | DNA protection during starvation protein | 6 | 14 | 58 | None |
| SAUSA300_1976 | Succinyl diaminopimelate desuccinylase | 10 | 10 | 24 | None |
| SAUSA300_0976 | Phosphoribosylamine-glycine ligase | 8 | 8 | 25 | None |
| SAUSA300_2462 | NAD(P)H-flavin oxidoreductase | 4 | 5 | 19 | None |
| SAUSA300_0491 | Cysteine synthase | 9 | 11 | 47 | None |
| SAUSA300_0965 | FolD | 6 | 4 | 19 | None |
| SAUSA300_1080 | FtsZ | 10 | 12 | 32 | None |
| SAUSA300_1367 | Cytidylate kinase | 3 | 3 | 16 | None |
| SAUSA300_1696 | d-Alanine aminotransferase | 6 | 5 | 22 | None |
| SAUSA300_0860 | Ornithine aminotransferase | 6 | 6 | 21 | None |
| SAUSA300_1880 | Glutamyl-tRNA amidotransferase | 6 | 6 | 15 | None |
| SAUSA300_0234 | Putative flavohemoprotein | 11 | 7 | 25 | None |
| SAUSA300_0135 | Superoxide dismutase | 6 | 9 | 43 | None |
| SAUSA300_2067 | Serine hydroxymethyltransferase | 7 | 8 | 21 | None |
| SAUSA300_0948 | Naphthoate synthase | 5 | 4 | 16 | None |
| SAUSA300_0141 | Phosphopentomutase | 6 | 5 | 18 | None |
| SAUSA300_1657 | Acetate kinase | 8 | 10 | 28 | None |
| SAUSA300_2187 | 30S ribosomal protein S5 | 5 | 12 | 42 | GRRFRF |
| SAUSA300_1725 | Transaldolase | 4 | 6 | 24 | None |
| SAUSA300_2091 | Purine nucleoside phosphorylase Deo-D type | 4 | 5 | 31 | None |
| SAUSA300_0009 | Serine-tRNA ligase | 5 | 8 | 18 | None |
| SAUSA300_1874 | Ferritin | 7 | 11 | 40 | None |
| SAUSA300_2202 | 50S ribosomal protein L23 | 4 | 5 | 46 | None |
| SAUSA300_2517 | Amidohydrolase family protein | 4 | 4 | 17 | None |
| SAUSA300_2463 | d-Lactate dehydrogenase | 7 | 7 | 20 | None |
| SAUSA300_1615 | Delta-aminolevulinic acid dehydratase | 3 | 3 | 15 | None |
| SAUSA300_2190 | 30S ribosomal protein S8 | 2 | 3 | 24 | None |
| SAUSA300_1139 | Succinyl-CoA synthetase, alpha subunit | 5 | 5 | 26 | GRKTRL |
| SAUSA300_0531 | 30S ribosomal protein S7 | 2 | 3 | 19 | None |
| SAUSA300_0672 | MarR family transcriptional regulator | 3 | 3 | 30 | None |
| SAUSA300_0688 | Oxidoreductase/aldo-keto reductase family | 6 | 6 | 23 | None |
| SAUSA300_1109 | Methionyl-tRNA formyl transferase | 5 | 5 | 21 | None |
| SAUSA300_0114 | SarS | 4 | 4 | 17 | None |
| SAUSA300_1653 | Metal-dependent hydrolase | 3 | 4 | 21 | None |
| SAUSA300_0916 | Conserved hypothetical protein | 6 | 6 | 37 | None |
| SAUSA300_0605 | SarA | 4 | 4 | 31 | None |
| SAUSA300_1442 | SrrA | 4 | 4 | 26 | THVKRL |
| SAUSA300_0380 | AhpC | 5 | 8 | 40 | AHKIKA |
| SAUSA300_2097 | Uncharacterized protein | 3 | 5 | 16 | None |
| SAUSA300_1494 | LipM | 3 | 3 | 17 | None |
| SAUSA300_1719 | Arsenate reductase | 2 | 2 | 20 | None |
| SAUSA300_1900 | Manganese-dependent inorganic pyrophosphatase | 4 | 4 | 17 | None |
| SAUSA300_1541 | GrpE | 3 | 3 | 17 | None |
| SAUSA300_1191 | Complement inhibitor | 2 | 2 | 27 | None |
| SAUSA300_0540 | HAD family hydrolase | 5 | 6 | 21 | None |
| SAUSA300_0173 | Uncharacterized protein | 2 | 2 | 17 | None |
| SAUSA300_0969 | PurS | 4 | 4 | 59 | None |
| SAUSA300_1131 | 30S ribosomal protein S16 | 3 | 3 | 49 | None |
| SAUSA300_1304 | Glyoxylase family protein | 3 | 3 | 18 | None |
| SAUSA300_2529 | PhnB | 2 | 2 | 19 | None |
| SAUSA300_2245 | SarR | 2 | 2 | 18 | None |
| SAUSA300_2315 | Lipoprotein | 2 | 2 | 19 | None |
| SAUSA300_1659 | Probable thiol peroxidase | 3 | 3 | 28 | None |
| SAUSA300_0015 | 50S ribosomal protein L9 | 2 | 2 | 17 | None |
| SAUSA300_2132 | Uncharacterized protein | 2 | 2 | 37 | None |
| SAUSA300_1358 | Nucleoside diphosphate kinase | 2 | 2 | 15 | None |
CoA, coenzyme A; HAD, haloacid dehalogenase.
The peptide count is the number of exclusive unique peptides that matched the identified protein across all MS samples.
The spectrum count is the number of counts of spectra that match different peptides (even if the peptides overlap), two different charge states of the same peptide, or both a peptide and a modified form of the peptide.
% coverage is the percentage of all the amino acids in the protein sequence that were detected in the sample.
Heparin-binding motif refers to the three linear heparin-binding motifs, namely, Cardin (XBBXBX), Weintraub (XBBBXXBX), and Sobel (XBBBXXBBBXXBBX).