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Analysis of CRISPR-Cas9 efficiencies in wild-type and NHEJ mutant backgroundsa
| Strain | Mean (SD) |
|
|---|---|---|
| No. of NATr colonies | % ade2 | |
| RSY426 (ku70 lig4) | 531 (514) | 81 (17) |
| RSY376 (ku70) | 306 (200) | 49 (37) |
| RSY281 (parent haploid) | 1,240 (1,144) | 25 (32) |
For this experiment, deletion constructs had long flanks (~1-kb homology flanking the ADE2 gene). n = 3 transformations per strain. The Cas9 and sgRNA are under a C. lusitaniae promoter.
