FIG. 7.

Reciprocal regulation of brown adipocyte and Wnt signaling genes in response to cold challenge or genetic obesity. (A) C57BL/6 mice were singly housed and maintained at room temperature (Control; n = 5) or at 4°C for 24 h (Cold exposure; n = 5). After euthanasia, interscapular tissues were dissected and RNA was prepared. Expression of UCP1, PGC-1α, Wnt10a, and Wnt10b was evaluated by quantitative PCR. Relative levels of RNA are shown graphically as the mean + standard deviation. Differences between treatments were evaluated by a Student's t test. An asterisk indicates statistical significance (P < 0.05). (B) RNA from interscapular tissue of wild-type (Wt) and ob/ob mice was isolated. Expression of UCP1, PGC-1α, Wnt10a, and Wnt10b mRNAs was evaluated by quantitative PCR, and relative expression is presented as the mean ± standard deviation. Differences between wild-type and ob/ob mice were evaluated by a Student's t test. An asterisk indicates statistical significance (P < 0.05).