FIG. 4.

Specific binding of NRF-2 to recognition sites within the hTFB1M and hTFB2M promoters. (A) Radiolabeled synthetic oligonucleotides containing COXIV, hTFB1M, or hTFB2M recognition sites were bound to an aliquot of partially purified nuclear extract that was eluted from heparin agarose at 0.25 M NaCl. The indicated DNA-protein complexes were separated by gel electrophoresis. The unlabeled competitor oligonucleotide or the supershifting antiserum added to the binding reactions is indicated above each lane. (B) The same radiolabeled oligonucleotides as in panel A were bound to either recombinant NRF-2α (rec α) or a mixture of recombinant NRF-2α and NRF-2β₁ (rec α + β₁). The indicated DNA-protein complexes were separated by gel electrophoresis. The unlabeled competitor oligonucleotide or the supershifting antiserum added to the binding reactions is indicated above each lane.