FIG. 5.

Mutational analysis of the hTFB1M promoter region. A series of 5′ deletions designed to progressively remove putative cis-acting elements was analyzed by gene transfection. The normalized luciferase activity obtained from the promoter fragment containing 489 nucleotides of 5′-flanking DNA (−489) was designated as 100%. Point mutations represented by X were introduced into the −489 promoter as described under Materials and Methods. The activities of all of the mutated promoters were expressed as a percentage of that obtained from the −489 promoter. Numbers represent the average ± standard error for three separate determinations.