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. 2017 Jun 14;108(6):1169–1176. doi: 10.1111/cas.13243

Figure 3.

Figure 3

Effect of nucleotide binding protein‐like (NUBPL) overexpression or knockdown on colorectal carcinoma cell migration and invasion. (a) Wound‐healing assay detecting the motility of SW480‐NUBPL cells. Columns: mean ± SD of triplicate experiments. *< 0.05, independent Student's t‐test. (b, c) Cell migration (b) and invasion (c) assays investigating the influence of NUBPL overexpression on SW480 and SW620 cells. Representative images of migrated or invaded cells are illustrated in the upper panels (magnification, ×100). Scale bar = 200 μm. Columns: mean ± SD of triplicate experiments. *< 0.05, independent Student's t‐test. (d, e) Effect of NUBPL knockdown on SW480‐NUBPL cell migration (d) and invasion (e) in Transwell assays. Examples of cells migrated through the PET‐membrane or Matrigel‐coated Transwell are shown in the left panel (magnification, ×100). Scale bar = 200 μm. Columns: mean ±SD of triplicate experiments. siNC, negative control siRNA. *< 0.05, independent Student's t‐test.