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. 2017 Jun 2;108(6):1135–1143. doi: 10.1111/cas.13234

Figure 6.

Figure 6

Epidermal growth factor (EGF) suppresses ferritin expression and increases labile iron pool (LIP) in CACO‐2 cells. (a) Ferritin, TfR1, p‐ERK and IRP2 expression in CACO‐2 cells co‐cultured with or without EGF (25 ng/mL). Recombinant ferritin protein used as a positive loading control to confirm antibody immunoreactivity (b). Ferritin concentration determined by ELISA in CACO‐2 cells co‐cultured with or without EGF (25 ng/mL). Data expressed as mean fold change in protein expression normalized to non‐treated control cells. Error bars denote ± SEM with * representing statistical significance when P < 0.05 and n = 6. (c) LIP changes in CACO‐2 cells co‐cultured with or without EGF (25 ng/mL). Data expressed as % change in fluorescence compared to non‐treated control cells. Error bars denote ± STD with P‐values representing statistical significance when P < 0.05 and n = 3. Arbitrary (arb.).