Skip to main content
. 2017 May 11;108(6):1263–1270. doi: 10.1111/cas.13233

Figure 2.

Figure 2

Location of the MET mutation found in familial EGFR‐mutant lung cancer. (a) Schematic representation of the MET protein. The transmembrane protein consists of the semaphorin‐like (Sema) domain, the plexin‐semaphorin‐integrin (PSI) domain, the immunoglobulin‐plexin‐transcription factor (IPT) 1–4 domains, the juxtamembrane (JM) domain, and the kinase domain. The precursor is cleaved into α‐chains and β‐chains, thus yielding the disulfide‐linked heterodimer. The NP_000236.2 protein was used as a reference for the positions. AA, amino acid. (b) Protein sequence alignment of MET (amino acid residues 371–390) in vertebrate species. Altered residues are colored red. The residues corresponding to the missense mutation found in familial EGFR‐mutant lung cancer are boxed. (c) The 3‐D structure of HGF‐MET complex. The modeling was generated using the Protein Data Bank (PDB) ID 1SHY; grey, HGF β‐chain; green, MET α‐chain; blue, Sema and PSI domains of MET β‐chain; orange, Asn375 in MET β‐chain. (d) Sanger sequencing chromatograms for MET (amino acid residues 371–380) in blood and tumor cells from the individual affected by familial EGFR‐mutant lung cancer. Blood cells from an unaffected control individual were used as a control.