FIG. 6.
Cell cycle analysis of cells partially depleted of histone H4. (A) DNA content and cell morphology of asynchronous cultures of the strains and at the conditions indicated in the legend to Fig. 1. Similar profiles were obtained with BYtetH4l-1C (data not shown). (B) Cell cycle progression of BYtetH4l-1C at either 5 (t::HHF2[5]) or 0.25 (t::HHF2[0.25]) μg of DOX/ml and of BY4741 at 5 μg of DOX/ml (wild type [wt]). DNA content of wild-type and t::HHF2 cells was determined by flow cytometry at the indicated times upon release from α-factor arrest in G1.