Table 1.

Real-time RT PCR of amniotic/allantoic fluids (AF) and chorioallantoic membranes (CAM) from embryonated chicken eggs inoculated with A/equine/Pulawy/2008

Dilution	10−5		10−6		10−7		10−8
Sample	AF	CAM	AF	CAM	AF	CAM	AF	CAM
Ct (n = 6)	19.3a	11.3	36	27.4	40	32.1	40	29.7
	19.3b	12.3	18.4a	8.5	40	32	40	34.6
	19.4a	12.2	19a	13.3	40	32.7	40	33.1
	14.5b	12.6	35.6	24.9	40	30.1	40	34.9
	16.3b	12.4	18.2a	8.9	40	33.9	38.9	34.2
	19.5a	11.5	35.9	24.3	40	30.8	40	32.4
Mean	18.05	12.05	27.18	17.88	40	31.93	39.81	33.15
SD	2.1	0.5	9.5	8.6	0	1.4	0.4	1.9
p		0.0050		0.1735		0.0028		0.0037

^aHA 64

^bHA 128

SD standard deviation

p - p value calculated with Mann–Whitney U test