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. 2005 Feb;25(4):1367–1378. doi: 10.1128/MCB.25.4.1367-1378.2005

FIG. 7.

FIG. 7.

Down-regulation of MEKK1(C) by Deltex due to proteasome-mediated degradation in T cells. (A) Inhibition of proteasome-mediated degradation restored the protein expression of MEKK1(C) in Deltex1-DO11.10 T cells. YFP- and deltex1-DO11.10 cells were untreated or treated with MG132 (50 μM) for 6 h and the levels of MEKK1(FL) and MEKK1(C) were determined by immunoblotting. (B) Ubiquitination of MEKK1(C) in Deltex1-expressing DO11.10 T cells but not in YFP controls. Total cellular extracts (250 μg) from panel A were immunoprecipitated using anti-MEKK1 antibody and protein-A Sepharose; the immune precipitates were resolved on SDS-PAGE and blotted with anti-ubiquitin (Ub) and anti-MEKK1(C) antibodies. IP, immunoprecipitation; WB, Western blot. (C) Ubiquitination of MEKK1(C) in Deltex-expressing normal T cells. Ubiquitination of MEKK1 in splenic T cells expressing Deltex1 or YFP were analyzed in the presence of MG132 as described for panel B.