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. 2005 Feb;25(4):1379–1388. doi: 10.1128/MCB.25.4.1379-1388.2005

FIG. 3.

FIG. 3.

MDA-MB-435 cells transiently transfected with Nm23-H1 and KSR1 also show increased Hsp90 binding to KSR1. MDA-MB-435 cells were transiently transfected with combinations of HA-KSR1 and either a control Flag vector or Nm23-H1-Flag, and subcellular fractions were prepared. (A) Western blot of lysates for cytoplasmic (α-tubulin) and nuclear (c-jun) markers, as well as endogenous and transiently transfected Nm23-H1. (B) Immunoprecipitation of HA-KSR1 from equivalent amounts of cytoplasmic lysates; coimmunoprecipitation of Hsp90 and Nm23-H1 was determined by Western blotting. (C) Immunoprecipitation of HA-KSR1 from equivalent amounts of nuclear lysates; coimmunoprecipitation of Hsp90 and HA-KSR1 was determined by Western blotting.