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. 2005 Feb;25(4):1379–1388. doi: 10.1128/MCB.25.4.1379-1388.2005

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FIG. 4. — KSR1 stability is decreased in Nm23-H1-overexpressing breast carcinoma cells exposed to cycloheximide. MDA-MB-435 cells were transiently transfected with pEGFP as a control for transfection efficiency, a HA-KSR1 vector, and either a control Flag or an Nm23-H1-Flag vector. Cells were then treated with 100 μM cycloheximide (a protein synthesis inhibitor) for various amounts of time. Lysates were analyzed by Western blotting with anti-HA, anti-Nm23-H1, and anti-GFP as a transfection control. Densitometric analysis of KSR1 expression normalized to GFP levels is graphed below.