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17-AAG disrupts Nm23-H1-KSR1-Hsp90 complex formation in H1-177 cells. Control C-100 and Nm23-H1-overexpressing H1-177 cells were transiently transfected with HA-KSR1 and exposed to 1 μM 17-AAG or DMSO (vehicle control) for 1 h. Cytoplasmic lysates were immunoprecipitated with anti-HA, and the binding of Hsp90 and Nm23-H1 proteins was determined by Western blotting.
