FIG. 7.

17-AAG preferentially inhibits the colonization of Nm23-H1 overexpressing MDA-MB-435 breast carcinoma cells. Equivalent cell numbers of control C-100, P96S mutant Nm23-H1 S-22 and wild type Nm23-H1 overexpressing H1-177 cell lines were plated in soft agar with semilog dilutions of 17-AAG. Colonies were counted three weeks later. Data represent the means ± SEM for triplicate cultures (for a representative experiment of n = 3), expressed as a percentage of vehicle control colonization for each line. C-100 and H1-177 colonization was significantly different: with 0.05 μM 17-AAG, P = 0.0046 (Student's t test).