FIG. 9.

Inherent transcriptional activity of MAGE-11 and the interaction domain for the AR FXXLF motif. Two-hybrid interaction assays were performed in HepG2 cells by transient transfection in 12-well plates with Effectene with 0.1 μg of 5XGAL4Luc3 reporter and 50 ng of the indicated GAL-MAGE-11 fusion protein vectors with either VP16 activation domain empty vector control (50 ng/well) (VP16-AD, top) or VP-AR1-660 (50 ng/well) (bottom). Luciferase activity and error were determined in the absence of hormone.