Skip to main content
. 2005 Feb;25(4):1425–1436. doi: 10.1128/MCB.25.4.1425-1436.2005

FIG. 2.

FIG. 2.

Hey1 represses AR-mediated transactivation in vivo. (A) COS-1 cells were cotransfected with 20 ng of TAT-GRE-E1B-LUC or Probasin-LUC and expression vectors for AR (5 ng) and Hey1 (10 ng). (B) COS-1 cells were cotransfected with 20 ng of TAT-GRE-E1B-LUC or Probasin-LUC and expression vectors for AR (5 ng) and Hey2 (10 ng). (C) COS-1 cells were cotransfected with 20 ng of TAT-GRE-E1B-LUC and expression vectors for Hey1 (10 ng) and PR (5 ng) or GR (5 ng), or with 10 ng of 2ERE-pS2-LUC and expression vectors for ERα (2.5 ng) and Hey1 (10 ng). After transfection, cells were washed and incubated for 24 h in the presence of vehicle (white bars) or a 10 nM concentration of hormones (gray bars; mibolerone for AR, 17β-estradiol for ERα, R5020 for PR, and dexamethasone for GR). Subsequently, cell lysates were assayed using a dual luciferase reporter system. Normalized values are expressed relative to the activity of AR, PR, GR, or ER alone in the presence of their respective ligands. The results shown represent the averages of results of at least two independent experiments assayed in quadruplicate + SD.