FIG. 6.

An active form of Notch (NICD) represses AR in vivo. (A) C2C12 cells were cotransfected with 20 ng of TAT-GRE-E1B-LUC and expression vectors for AR (5 ng), NICD (10 ng), and Hey1 (10 ng). (B and C) MCF-7 cells were cotransfected with 20 ng of TAT-GRE-E1B-LUC and expression vectors for NICD (0.1, 1, or 5 ng). (D and E) MCF-7 cells were cotransfected with 20 ng of TAT-GRE-E1B-LUC and expression vectors for Hey1 (10 or 20 ng). After transfection, cells were washed and incubated in fresh medium for 16 h. Subsequently, cells were incubated for 24 h with vehicle (white bars), 10 nM mibolerone (gray bars), or 10 nM R5020 (black bars). Cell lysates were then assayed using a dual luciferase reporter system. Data are presented as described for Fig. 2. The results shown represent the averages of results from at least two independent experiments assayed in quadruplicate + SD.