FIG. 10.

Hypothetical modes of membrane association of DMPK isoforms. A schematic representation of the putative association of DMPK isoforms with their target membrane is shown. C-terminal DMPK anchors can either pass the membrane entirely (mode I) or give close surface binding to the outer leaflet of the lipid bilayer (mode II). A requirement of the coiled-coil domain for proper MOM localization of mDMPK C suggests that either heterologous protein interactions may stabilize mDMPK localization at the membrane (mode III) or multimerization of DMPK via the coiled-coil domain may promote binding specificity and/or strength (mode IV). Possible intramolecular effects of proximity of the coiled-coil segment on folding of the hydrophobic domain can also be envisaged but are not included in the figure.