Fig. 3.

a 864669, b 4248543, and c 856350 do not inhibit JAKs in Cal33 cells. Cal33 cells were serum starved and treated with increasing concentrations of 864669, 4248543, and 856350, respectively. At the end of 2 h and 45 min, cells were stimulated with IL6 (50 ng/ml,15 min) and harvested and protein lysates were prepared and ran on a 10% SDS-polyacrylamide gel to determine the expression of pJAK1, JAK1, pJAK2, JAK2, pJAK3, and JAK3. β-Tubulin was used as a loading control. 864669 (Fig. 3a), 4248543 (Fig. 3b), and 856350 (Fig. 3c) do not inhibit any of the JAKs at the concentration tested. Results are from three separate experiments