Figure 5.

MCC Is a Functional Target Gene of miR-4261 in HCT116 Cells

(A) MCC siRNAs take effect in HCT116 cells (n = 4). (B–D) MCC siRNAs abolish the suppressive effect of miR-4261 inhibitor on cell proliferation, G1/S phase transition of cell cycle, and cell migration in HCT116 cells, as determined by (B) EdU incorporation assay (n = 4; scale bars, 50 μm), (C) flow cytometry (n = 4), and (D) transwell assay (n = 3; original magnification ×200). EdU is represented by green and DAPI by blue. *p < 0.05 versus control; ^#p < 0.05 versus miR-4261 inhibitor. Data are presented as mean ± SEM.