Figure 5.

Effects of BMN 250 on Neuro-inflammation and Reactive Astrocytosis at 28 Days following Four 100 μg ICV Doses over 2 Weeks

(A) Representative images from the cingulate cortex of immunohistochemical signals (white) for IBA1, CD68, and GFAP. (B) Quantification of total microglia²⁹ (IBA1-positive foci). (C) Quantification of actively phagocytic microglia30, 31 (percent CD68-positive/IBA1-positive) and astrocytosis (percent GFAP-positive area) in the cingulate cortex, thalamus, and entorhinal cortex (mean ± SEM). VEH, vehicle. n = 6–8 animals per treatment group. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.