TABLE 2.
PCR primers used in this study
| Namea | Target gene | Sequence |
|---|---|---|
| rRNA1 | 18S rRNA | 5′-GTGAAACTGCGAATGGCTCA-3′ |
| rRNA2 | 18S rRNA | 5′-CCAACTTTCCGGCTCTGGGG-3′ |
| alp1 | Alkaline protease | 5′-AAACGCAATCTGGAGCGTCG-3′ |
| alp2 | Alkaline protease | 5′-CATTGCCATTGTAGGCAAGC-3′ |
| alp3 | Alkaline protease | 5′-ATTCCTGGCAAGTACATCGTGACCTTCAAG-3′ |
| alp4 | Alkaline protease | 5′-ATTGCCATTGTAGGCAAGCTTGTTGGGGCT-3′ |
| benA1 | β-Tubulin | 5′-AATAGGTGCCGCTTTCTGG-3′ |
| benA2 | β-Tubulin | 5′-AGTTGTCGGGACGGAAGAG-3′ |
a
Primer rRNA1 corresponds to nucleotides 43 to 62 and primer rRNA2 is complementary to nucleotides 1669 to 1688 in the 18S rRNA gene of A. fumigatus (11). Primer alp1 corresponds to nucleotides 480 to 499 and primer alp2 is complementary to nucleotides 1676 to 1695 in the Alp gene of A. fumigatus (6). Primer alp3 corresponds to nucleotides 393 in the Alp protease gene of A. fumigatus (6). Primers benA1 and benA2 are described in Geiser et al. (3).