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To determine the respective sensitivities of each assay, serial dilutions of pGEMHS1 and pGEMHS2 plasmid DNAs were amplified by the HSV-1- and HSV-2-specific LAMP, respectively. The detection of LAMP products was assessed by agarose gel electrophoresis and turbidity assay using an LA-200. Marker, 123-bp DNA ladder marker.
