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letter
. 2017 Jun 16;13:1168–1173. doi: 10.3762/bjoc.13.115

Figure 1.

Figure 1

Comparative analysis of hapalindole-type alkaloids and their BGCs in two welwitindolinone producers implicates the functional role of Fe/2OG-dependent WelO5* halogenase in structural diversifications in H. welwitschii IC-52-3. a) Representative hapalindole-type alkaloids tabulated based on oxidation states in the welwitindolinone producers H. welwitschii UTEX B1830 and IC-52-3. b) Relative quantities of 1 and its biogenetic derivative 1a versus 2 and its biogenetic derivatives (2a, 3–6) in H. welwitschii IC-52-3 and UTEX B1830. c) Comparison of the ratio of chlorinated versus dechlorinated hapalindole-type alkaloids based on their biosynthetic origins (i.e., 1 or 2) in H. welwitschii IC-52-3 and UTEX B1830. d) Comparison of welwitindolinone BGCs in H. welwitschii IC-52-3 and UTEX B1830. The tailoring enzyme coding genes, including welU1-3, welO1-5 and welM, encoded in the welwitindolinone BGC from H. welwitschii IC-52-3, were renamed due to their extreme similarities to those from H. welwitschii UTEX B1830. e) Sequence comparison of WelO5 and WelO5*. Varied residues are highlighted in red with the most aggregated region 215-232 in grey rectangle.