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In vitro characterization of a WelO5 variant with enhanced specificity towards 1. All of the HPLC data shown are in vitro assays conducted with WelO5* or its variants (20 µM) with 0.5 mM of 1 or 2 for 20 min at 30 °C. HPLC was run with a C18 Luna column (Phenomenex 250 × 4.6 mm) with a solvent gradient from 60–90% methanol/water in 30 min at a flow rate 1 mL/min.
