Skip to main content
. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Mol Microbiol. 2017 Jan 26;103(5):860–874. doi: 10.1111/mmi.13594

Figure 3.

Figure 3

Complementation of sepA mutant by plasmid and exogenous protease. Strains were grown statically for 20 hours in 48-well microtiter plates. With the exception of (C), oneway ANOVA with multiple comparisons (Bonferroni correction) was performed. * indicates p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001. A. Microtiter biofilm assay using strains complemented with psepA. Results are pooled from three experiments with three replicates each. B. Microtiter biofilm assay with Aureolysin (Aur) added to complement 1457ΔicaΔsepA. Results are pooled from two experiments with three replicates each. C. Microtiter biofilm assay with Aur added to 1457Δica and 1457ΔicaΔaap. One representative experiment is shown. Two-way ANOVA with multiple comparisons (Bonferroni correction) was performed. D. Microtiter biofilm assay using strains complemented with psepA. Results are pooled form three experiments with three replicates each.