. Author manuscript; available in PMC: 2018 Mar 1.

Published in final edited form as: Mol Microbiol. 2017 Jan 26;103(5):860–874. doi: 10.1111/mmi.13594

Table 1. qPCR analysis of protease expression in S. epidermidis.

	6 h		18 h

	Fold regulation^a (CI)	p-value^b	Fold regulation^a (CI)	p-value^b
Δ ica versus ΔicaΔsarA
sepA	22.3 (19.1 – 26.1)	<0.0001	7.8 (2.2 – 27.2)	0.01
ecp	-2.2 (-11.1 – 2.2)	0.24	2.7 (-1,4 – 10.2)	0.1
esp	1.9 (-1.1 – 4.1)	0.07	6.3 (2.4 – 16.9)	0.006

**ΔicaΔsarA versus ΔicaΔsarAΔecp**
sepA	1.0 (-1.9 – 2.0)	0,99	1.6 (-1.1 – 2.9)	2.3

**ΔicaΔsarA versus Δ icaΔsarAΔesp**
sepA	-1.39 (-2.6 – 1.3)	0.22	1.3 (-4.9 – 8.8)	0.69

calculated using the 2^ΔΔCT method (Livak & Schmittgen, 2001)

two-tailed Student's t-test