Figure 2.

Identification of a Minimal, High-Affinity VERL/Lysin Complex

(A–C) Coomassie-stained SDS-PAGE analysis of pull-down experiments of lysin co-transfected with different His-tagged VERL constructs. Lane 1 in (A) is a refolded lysin control. Open and closed circles in (C) indicate VR3 isoforms resulting from alternative signal peptide cleavages (see STAR Methods).

(D) SEC analysis of purified VR1+ (top) and VR3 (middle) by themselves and mixed with lysin^R in a 1:2 ratio. Peaks are normalized to 80 mAU. Bottom: SDS-PAGE analysis of SEC peak fractions.

(E) MST analysis of lysin^R interaction with VR1+, VR3, and negative control ZP2 ZP-N1. Results are shown as mean ± SD.

See also Figure S2.