Figure 5.

Liver Gluconeogenesis Is Critical to Establish Disease Tolerance to Sepsis

(A) G6pc1 protein expression in the liver of control (G6pc1^lox/lox; Ctr) and Alb^CreER^T2G6pc1^Δ/Δ (G6pc1^Δ/Δ) mice.

(B) Blood glucose levels of G6pc1^lox/lox (n = 12) and Alb^CreER^T2G6pc1^Δ/Δ (n = 12) mice subjected to CLP. Data are shown as mean ± SEM from three independent experiments.

(C) Survival of control (G6pc1^lox/lox; n = 10) and Alb^CreER^T2G6pc1^Δ/Δ (n = 10) mice subjected to CLP. Data were pooled from two independent experiments with similar trend.

(D) Blood glucose of control (C57BL/6; n = 7) and Alb^CreER^T2G6pc1^Δ/Δ (n = 5) mice subjected to PCI. Data are shown as mean ± SD from two independent experiments with the same trend.

(E) Survival of control (C57BL/6; n = 10) and Alb^CreER^T2G6pc1^Δ/Δ (n = 8) subjected to PCI. Data pooled from three independent experiments with similar trend.

(F) CFU for aerobic (Ae) and anaerobic (An) bacteria, 6 hr after PCI. Red bars represent mean values and dotted circles represent individual mice. PC, peritoneal cavity.

(G) Blood glucose levels in G6pc1^lox/lox (n = 6) and Alb^CreER^T2G6pc1^Δ/Δ (n = 7) mice receiving heme (i.p. 30 mg/kg BW). Mean ± SD from two independent experiments.

(H) Survival of same mice as (G).

(I) Blood glucose levels in G6pc1^lox/lox (n = 6) and Alb^CreER^T2G6pc1^Δ/Δ (n = 9) mice receiving LPS (i.p. 5 mg/kg BW). Mean ± SD from two independent experiments.

(J) Survival of same mice as (I).

(K) Blood glucose levels in G6pc1^lox/lox (n = 9) and Alb^CreER^T2Gpc1^Δ/Δ (n = 10) mice receiving poly(I:C) (intra-retro orbital [i.r.o.] 30 mg/kg BW). Mean ± SD from two independent experiments.

(L) Survival of same mice as (K).

Numbers in gray (B, D, G, I, and K) are live/total mice at each time point. ^∗p < 0.05; ^∗∗p < 0.01.

See also Figure S6.