Figure 7.

Antioxidants Bypass the Requirement of FTH in the Establishment of Normoglycemia and Disease Tolerance to Sepsis

(A–C) Relative weight (A), blood glucose (B), and survival (C) of Mx1^CreFth^Δ/Δ mice subjected to CLP and receiving NAC (n = 7; 15 mg/kg) or vehicle (n = 6). Data pooled from two independent experiments.

(D) Colony forming units (CFU) for aerobic (Ae) and anaerobic (An) bacteria, 48 hr after CLP. PC, peritoneal cavity. Red bars represent mean values and doted circles represent individual mice. Pooled from two independent experiments.

(E) Expression of G6PC1 mRNA quantified by qRT-PCR in HepG2 cells exposed (+) or not (−) to heme, TNF, and/or NAC. Data shown as mean ± SD from four independent experiments.

(F and G) Blood glucose (F) and survival (G) of Mx1^CreFth^Δ/Δ mice subjected to CLP and receiving BHA (n = 11; 50 mg/kg) or vehicle (n = 9). Data shown as mean ± SD. Pooled from three independent experiments.

(H–J) Relative weight (H), blood glucose (I), and survival (J) of Alb^CreER^T2G6pc1^Δ/Δ mice subjected to CLP and receiving NAC (n = 5) or vehicle (n = 5). Mean ± SD from one experiment.

NS, non-significant. ^∗p < 0.05, ^∗∗p < 0.01. Numbers in gray (A, B, F, I, and J) indicate live/total mice.