Figure S3.

FTH Does Not Modulate Cardiovascular Function, Related to Figure 1

(a) H&E stained paraffin section of hearts from Fth^lox/lox and Mx1^CreFth^Δ/Δ mice before and 48 hr after CLP. Images are representative of at least 3 mice per genotype per experimental conditions. Magnifications are 100 (top) and 400x. (b) Cardiac pressure-volume (PV) loop analysis of Fth^lox/lox and Mx1^CreFth^Δ/Δ mice, 48 hr after CLP. Load-dependent, i.e. Heart rate (HR) end-systolic pressure (ESP), cardiac output (CO), relaxation (Tau-Glantz), pressure volume area (PVA) and mean arterial pressure (MAP) and load-independent, i.e. slope of end-systolic pressure volume Relationship (ESPVR) and maximum of generated energy (E_max) parameters are shown as mean ± SD from n = 3-6 mice per group pooled from 4 independent experiments. NS: non-significant. (c) Vascular leakage monitored by quantification of Evans Blue accumulation in parenchyma organs, before and 48 hr after CLP. Data are shown as mean ± SD from n = 5 mice per group, pooled from 3 independent experiments. NS: non-significant.