Skip to main content
. Author manuscript; available in PMC: 2018 Mar 22.
Published in final edited form as: Neuron. 2017 Mar 22;93(6):1464–1479.e5. doi: 10.1016/j.neuron.2017.02.034

Figure 4. Inhibition of genetically defined CeA neurons during feeding, drinking, and defensive behaviors.

Figure 4

(A–G) Behavioral assessment of percent feeding during food presentation in food-deprived mice (first column); percent drinking during water presentation in water-deprived mice (second column); percent freezing during presentation of footshocks on Day 1 (third column) and contextual recall without optogenetic inhibition on Day 2 (fourth column) from optogenetic inhibition of CeC Prkcd+ (n = 8, 8) (A), CeL Prkcd+ (n = 10, 8) (B), CeL Sst+ (n = 8, 8) (C), CeL Crh+Nts+Tac2+ (n = 8, 8) (D), CeM Nts+ (n = 9, 8) (E), CeM Sst+ (n = 8, 8) (F), and CeM Tac2+ (n = 9, 8) (G) neurons. All animals underwent the feeding test, followed by the drinking test, followed by contextual fear conditioning. Representative histology of eArch 3.0 expression and optic fiber placement in the targeted CeA neurons (fifth column). eArch 3.0 expression was pseudocolored in correspondence with selected color scheme (Figure 1P). Significance for unpaired t-test, *P< 0.05, **P<0.01, ***P<0.001, sample size (n = experimental,control) (A–G). Anterior-posterior (AP) distance from Bregma (mm), scale bar, 250 μm.